Journal of the American Chemical Society, Vol.126, No.45, 14899-14909, 2004
Electrochemical potential-step investigations of the aerobic interconversions of [NiFe]-hydrogenase from Allochromatium vinosum: Insights into the puzzling difference between unready and ready oxidized inactive states
Dynamic electrochemical studies, incorporating catalytic voltammetry and detailed potential-step manipulations, provide compelling evidence that the oxidized inactive state of [NiFe]-hydrogenases termed Unready (or Ni-A) contains a product of partial reduction of O-2 that is trapped in the active site.