Pseudomonas fluorescens KUAF-68, which was isolated from Antarctica, had both ice-nucleating protein and antifreeze protein activities in the culture broth. We found that both proteins were separately produced based on the results of column chromatography, SDS-PAGE analysis and Southern hybridization. The activity of the ice-nucleating protein was stimulated by the addition of glycine (0.020N%), whereas the activity of the antifreeze protein was stimulated by the addition of L-asparagine (0.025N%). This is the first report on the production of two types of ice crystal-controlling proteins in one bacterial strain.