Journal of Bioscience and Bioengineering, Vol.98, No.4, 304-305, 2004
Cell culture in a closed nano-space
The minimum size of a closed nano-space in which cells can survive was determined using 4-nl nanowells. One or two cells could divide in the nanowell. Our results suggest that the cell division activity in the nano-space is determined by the conflict between intercellular effects and consumption of substrates.