The effect of decreasing oxygen feed rates on the growth and metabolism of Torulaspora delbrueckii and Saccharomyces cerevisiae in chemostat cultures was investigated. The biosynthetic oxygen requirement, i.e. the minimum specific oxygen consumption rate required for steady-state growth at a dilution rate of 0.10 h(-1), of T. delbrueckii was quantified to be less than 0.1 mmol O-2 g(-1) h(-1). Under strict anaerobiosis, washout of T. delbrueckii occurred, whereas for S. cerevisiae it did not. Under oxygen-limited conditions, the increase in fermentative ability of T. delbrueckii with diminishing oxygen supply was less pronounced than that of S. cerevisiae. These results indicate that T. delbrueckii was more disturbed in its energy balance than S. cerevisiae under strict anaerobiosis, and they may explain why T. delbrueckii exhibits poorer growth than S. cerevisiae under this condition.