화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.29, No.3, 294-299, May, 2005
Export Citation
온도응답성 고분자의 패턴상 그래프트를 이용한 공배양법
Cell Co-culture Method by Patterned Graft of Thermo-Responsive Polymer
배진숙, 안창현, 윤관한, 권오형†, 강인규1, MasayukiYamato2, AkihikoKikuchi2, TeruoOkano2
  • 금오공과대학교 고분자공학과
  • 1경북대학교 고분자공학과
  • 2동경여자의과대학 선단생명의과학연구소
Jin Suk Bae, Chang Hyun Ahn, Kwan Han Yoon, Oh Hyeong Kwon†, Inn-Kyu Kang1, Masayuki Yamato2, Akihiko Kikuchi2, and Teruo Okano2
  • Department of Polymer Science and Engineering, Kumoh National Institute of Technology, 1 Yangho-dong, Gumi, Gyeongbuk 730-701, Korea
  • 1Department of Polymer Science, Kyungpook National University, 1370 Sankyuk-dong, Puk-ku, Daegu 702-701, Korea
  • 2Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan
E-mail:
초록
온도응답성 고분자인 PIPAAm을 포토마스크를 사용하여 전자빔조사에 의해 패턴상으로 세포배양용 폴리스티렌 접시표면에 그래프트하였다. 폴리스티렌 표면에의 PIPAAm의 그래프트는 ATR-FTIR과 ESCA에 의한 표면분석을 통해 확인하였다. 이러한 표면에 간실질세포를 37 ℃에서 배양하였고, 균일하게 간세포가 배양된 배양접시를 PIPAAm의 LCST 이하인 20 ℃로 배양온도를 낮추어 PIPAAm이 그래프트된 도메인에 접착된 간실질세포를 탈착시키고 배양 접시를 다시 37 ℃로 올린 후 두 번째 혈관내피세포를 파종하여 PIPAAm이 그래프트된 도메인에만 선택적으로 접착시킴으로써 같은 평면상에서 간실질세포와 혈관내피세포를 공배양할 수 있게 되었다. 이러한 방법으로 생체외에서 간실질세포와 혈관내피세포를 장기간에 걸쳐 공배양할 수 있었다.
Thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) was covalently patterned by masked electron beam irradiation. Introduction of PIPAAm on tissue culture polystyrene dish was confirmed by ATR-FTIR and ESCA measurements. Hepatocytes were cultured at 37 ℃ on these surfaces. Cells adhered on PIPAAm-grafted domains were detached by reducing culture temperature to 20 ℃. Endothelial cells were then seeded and cultured on the same surfaces. Seeded endothelial cells were selectively attached on hepatocytes detached and PIPAAm-grafted domains and could be co-cultured with hepatocytes on the same culture dishes with clear pattern. This co-culture method enabled long-term co-culture of hepatocytes with endothelial cells.
Keywords:patterning;thermo-responsive polymer;co-culture;endothelial cell;hepatocyte
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