화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.13, No.3, 257-264, June, 2005
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Immobilization of Lactobionic Acid on Polyurethane Films and Their Interaction with Hepatocytes
Wan Meng1, 2, Kyung-Hye Jung3, Inn-Kyu Kang3, †, Oh Hyeong Kwon4, and Toshihiro Akaike5
  • 1Department of Chemical Engineering and Polymer Science, Yanbian University, Yanji, 133002, China
  • 2Department of Polymer Science, Kyungpook National University, Daegu 702-701, China
  • 3Department of Polymer Science, Kyungpook National University, Daegu 702-701, Korea
  • 4Department of Polymer Science & Engineering, Kumoh National Institute of Technology, Gumi 730-701, Korea
  • 5Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama 227, Japan
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Polyurethanes containing z-lysine segments in the main chain (PULL) were synthesized from 4,4'-diphenylmethyl diisocyanate, poly(tetramethylene glycol), and z-lysine oligomer as a chain extender. The PULL film was treated first with a 10% HBr-acetic acid solution and subsequently with a saturated sodium bicarbonate aqueous solution to produce a primary amine group on the surface (PULL-N). Lactobionic acid (LA).immobilized PULL (PULL-L) was prepared by the coupling reaction of the PULL surface amine groups and the LA carboxylic acid groups. The surface-modified PULLs were then characterized by attenuated total reflection-Fourier transform infrared spectroscopy, electron spectroscopy for chemical analysis, atomic force microscopy, and contact angle goniometry. In the hepatocytes adhesion experiment, the cells poorly adhered to the PULL surface, although they adhered moderately well to the PULL-N surface. On the other hand, the cells adhered well to the PULL-L surface, suggesting the good affinity of the surface β-galactose moieties for hepatocytes. When hepatocytes were cultured in the presence of epidermal growth factor for 48 h, the cells rapidly aggregated on the PULL-L surface, whereas they aggregated only slowly on the other surfaces. The PULL prepared in this study has the potential to be used as a coating material for the enhancement of hepatocyte adhesion.
Keywords:films;polyurethanes;surfaces;hepatocytes
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