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Journal of Bioscience and Bioengineering, Vol.99, No.3, 300-302, 2005
Static suspension culture of carrot somatic embryos
Three culture methods of carrot somatic embryos were compared: conventional shake flask culture, static suspension culture using Erlenmeyer flasks, and static suspension culture using petri dishes. Compared with the former two cultures, the last one resulted in an enhanced ratio of torpedo-shaped embryos and suppressed cotyledonary-stage embryo formation.