주조된 매크로 다공성 Poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) 막대를 이용한 카페인과 트립토판의 분리

김룡매; 염홍원; 노경호; Longmei Jin; Hongyuan Yan; Kyung Ho Row

Korean Chemical Engineering Research, Vol.43, No.5, 603-608, October, 2005

Separation of Caffeine and Tryptophan Using Molded Macroporous Poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) Rods

김룡매, 염홍원, 노경호 ^†

인하대학교 화학공학과, 초정밀생물분리기술연구센터, 402-751 인천시 남구 용현동 253

Longmei Jin, Hongyuan Yan, and Kyung Ho Row^†

Center for Advanced Bioseperation Technology and Department of Chemical Engineering, Inha University, 253, Younghyun-dong, Nam-gu, Incheon 402-751, Korea

E-mail:

초록

주조된 매크로 다공성 poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) 막대를 간단한 주형 과정을 거쳐서 실험실 내 튜브모양의 주형인 크로마토그래피 칼럼(4.6×100 mm)을 사용하여 자유 라디칼 중합에 의해 만들었다. 이는 에폭시 그룹이 유도된 일체형 칼럼과 황산을 이용한 에폭시드 그룹의 화학적인 변형과정을 이용하였다. 단량체 및 포로겐(세공을 생성하는 물질)의 비율, 온도 등 중합반응의 조건을 변화시켜서 세공크기를 조절하여 물질의 체류시 간에 영향을 미쳤다. 본 연구에서는 주조된 일체형 칼럼을 이용하여 카페인과 트립토판을 분리하였으며, 중합원료조성 이 일체형 칼럼의 효율과 선택도 그리고 분리도에 미치는 영향을 고찰하였다.

The molded macroporous poly (glycidyl methacrylate-co-ethylene glycol dimethacrylate) rods produced by a facile molding process were polymerized in situ within a tubular mold, chromatographic column (4.6×100 mm) by free radical polymerization. It was complemented by epoxy derivatized monolithic column and chemical modification of the epoxide groups with the sulphuric acid. By variation of the polymerization conditions, such as the ratio of the monomers, the porogen (pore generating material), and the temperature, the pore size could be varied, so the retention time of the samples may be adjusted. For the mixture of caffeine and tryptophan in the prepared monolithic column, the influences of polymerization material compositions to the efficiency, selectivity, and resolution of the monolithic column were investigated.

Keywords:Separation;Macroporous Monolithic Column;Polymerization;Modification

[References]

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[Related Articles]

[1] Svec F, J. Sep. Sci., 27(10), 747 (2004)

[2] Unger KK, Packings and Stationary Phases in Chromatographic Techniques, M. Dekker, NY, USA (1990)

[3] Svec F, Tennikova TB, Deyl Z, "Monolithic Materials: Preparation, Properties, and Applications", Elsevier, Amsterdam, 173 (2003)

[4] Krajnc P, Leber N, Stefanec D, Kontrec S, Podgornik A, J. Chromatogr. A, 1065(1), 69 (2005)

[5] Ikegami T, Tanaka N, Curr. Opin. Chem. Biol., 8(1), 1 (2004)

[6] Strancar A, Podgornik A, Barut M, Necina R, in: R. Freitag (Ed.), Advances in Biochemical Engineering/Biotechnology (Modern Advances Chromatography), vol. 76, Springer, Heidelberg, 49 (2002)

[7] Azanova VV, Hradil J, Svec F, Pelzbauer Z, Panarin EF, React. Polym., 12(3), 247 (1990)

[8] Azanova VV, Hradil J, Sytov GP, Panarin EF, Svec F, React. Funct. Polym., 16(1), 1 (1991)

[9] Hradil J, Svec F, React. Funct. Polym., 13(1), 43 (1998)

[10] Luo Q, Zou H, Xiao X, Guo Z, Kong L, Mao X, J. Chromatogr. A, 926(2), 255 (2001)

[11] Gong B, Ke C, Geng X, Chinese J. Chem., 22(3), 283 (2004)

[12] Calleri E, Massolini G, Lubda D, Temporini C, Loiodice F, Caccialanza G, J. Chromatogr. A, 1031(1), 93 (2004)

[13] Wu N, Dempsey J, Yehl PM, Dovletoglou A, Ellison D, Wyvratt J, Anal. Chim. Acta, 523(2), 149 (2004)

[14] Gritti F, Piatkowski W, Guiochon G, J. Chromatogr. A, 983(1), 51 (2003)