The electrophoretic behavior of a biocolloid covered with a charged membrane is theoretically analyzed in the present study. Here, the influences of nonuniformly distributed fixed groups, absorption of cations by fixed original functional groups, variation in dielectric constant in the electrophoretic system, and ionic sizes are considered. The results of numerical simulation suggest that a larger absolute value of the electrophoric mobility of biocolloids could be generated by larger membrane electricity. The absolute value of the electrophoric mobility for the nonlinear distribution of the fixed groups is larger than that for the linear distribution of the fixed groups. The absolute value of the electrophoric mobility increases with (1) the concentration of total fixed groups, (2) the cation-absorption equilibrium constant, (3) the nonuniform feature index for functional-groups distribution, (4) the dielectric constants of the inner uncharged membrane zone for only mobile cationic charge and for both mobile cationic and anionic charge, and (5) the effective size of the cations. An increase in the absolute value of the electrophoric mobility can also be resulted from a decrease in the following parameters: (1) the friction coefficient of the biocolloidal membrane phase, (2) the membrane thickness, (3) the dielectric constant of space for all charge and of outer uncharged membrane zone, (4) the effective sizes of anions and fixed groups, and (5) the number of cations and the fixed original functional groups involved in the formation of a cation-functional group complex.