The objective of this study was to investigate the relationship of the froth flotation performance in removal of emulsified ethylbenzene in water with microemulsion formation and with foam formation characteristics. The surfactant used was dihexyl sulfosuceinate (Aerosol MA or AMA) which can form microemulsions with ethylbenzene. The systems studied were designed to form Winsor Type III microemulsions with ethylbenzene, which generally correspond to ultra-low interfacial tensions between oil and water phases. By varying the surfactant concentration, NaCl concentration, and oil-to-water ratio, it was found that the lowest interfacial tension was obtained at 1 wt% AMA and 3 wt% NaCl, while the interfacial tension was not substantially influenced by the oil-to-water ratio. The highest oil removal was achieved in froth flotation with 0.3 wt% AMA and 3 wt% NaCl. No separation was experienced when the NaCl concentration exceeded 4 wt% due to the poor foamability of the froth formed under these conditions. Therefore, these results demonstrate that both interfacial tension and foam characteristics influence the efficiency of oil removal in the froth flotation process.