An efficient fedbatch process for the production of Lactobacillus kefir DSM 20587 cells was developed. An improvement in space time yield of 270% ( 3.7 g(DCW) l(-1)-day(-1)) and in final enzyme activity of 440% (9.1 U/ml) was achieved on a 150 l scale by controlling the oxygen transfer rate to 7 - 9 mmol l(-1) h(-1). The cells exhibited good and highly stereoselective reducing activities against tert-butyl 6-chloro-3,5-dioxohexanoate. tert-Butyl (3R, 5S)-6-chlorodihydroxyhexanoate,a chiral building block for HMG-CoA reductase inhibitor synthesis, was produced with 47.5% yield and > 99% ee at C(3)3 and C(5)5 in a simple batch biotransformation process.