By using our previously optimized media and a fed-batch operation controlled by LabVIEW Software, the key parameter for a high production of alkaline protease using the marine bacterium, Teredinobacter turnirae, was to maintain a low concentration of C and N-sources ( < 2 g sucrose l(-1) and < 0.2 g NH4Cl l(-1)) using an appropriate fed-batch culture system. A maximum protease activity of 8250 U ml(-1) was thus achieved.