Modeling of protein adsorption to DEAE sepharose FF: Comparison of data with model simulation

Mohammad Reza Aboudzadeh; Zhu Jiawen; Wu Bin

Korean Journal of Chemical Engineering, Vol.23, No.1, 124-130, January, 2006

DOI10.1007/BF02705703 Export Citation

Modeling of protein adsorption to DEAE sepharose FF: Comparison of data with model simulation

Mohammad Reza Aboudzadeh^†, Zhu Jiawen¹, and Wu Bin¹

Nuclear Research Center for Agriculture & Medicine, AEOI, P.O. Box 31585-4395, Karaj, Iran
¹Chemical Engineering Research Center, East China University of Science and Technology, Shanghai, 200237, China

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The equilibrium and kinetic characteristics of the adsorption of human serum albumin (HSA) and ovalbumin (OVA) to the DEAE Sepharose FF weak anion exchanger were experimentally determined. The rate for protein adsorption was simulated with two different models, the first being based on a single lumped kinetic parameter, while the second includes the individual mass transfer processes occurring prior to the adsorption intervention, i.e., diffusion across the liquid film surrounding individual particles and diffusion within the ion exchanger particle itself. The actual adsorption of OVA to DEAE Sepharose FF in fully mixed stirred vessels and in packed bed columns was consistent with both models. In the case of HSA, however, the adsorption profile in an agitated vessel was consistent only with the pore diffusion model and neither model could correctly predict the latter part of the breakthrough profile observed in packed bed experiments.

Keywords:Ion Exchange Chromatography;Breakthrough;Stirred Vessel;Packed Bed;Langmuir;HSA;OVA

[References]

Aboudzadeh MR, Jiawen Z, Wu B, Chinese J. Biomedical Eng., 13(4), 166 (2004)
Baker J, Finite element computational fluid mechanics, McGraw-hill, New York (1983)
Bohart GS, Adams EQ, J. Am. Chem. Soc., 42, 523 (1920)
Chen WD, Dong XY, Bai S, Sun Y, Biochem. Eng. J., 14, 45 (2003)
Chung SF, Wen CY, AIChE J., 14, 857 (1968)
Craig LC, J. Biol. Chem., 155,519, 1944, citied in Guichon, G. and Lin, B., Modeling for preparative chromatography, Academic press (2003)
Danckwerts PV, Chem. Eng. Sci., 2, 1 (1953)
De Vault D, J. Am. Chem. Soc., 65(4), 532 (1943)
Foo SC, Rice RG, AIChE J., 21, 1149 (1975)
Foster JF, Rosenoer VM, Oratz M, Rothschild MA, Albumin structure, function and uses, Pergamon Press, 53 (1977)
Geankoplis CJ, Transport processes and unit operations, Allyn and Bacon, MA, 2nd Ed. (1983)
Guiochon G, Golshan-Shirazi S, Jaulmes A, Anal. Chem., 60, 1856 (1988)
Guichon G, Shirazi SG, Katti AM, Fundamentals of preparative and nonlinear chromatography, Academic Press, Boston, MA (1994)
Hirs CHW, Moore S, Stien WH, J. Am. Chem. Soc., 73, 1893 (1951)
Huckman ME, Latheef IM, Anthony RG, AIChE J., 47(6), 1425 (2001)
Kaczmarski K, Antos D, Sajonz H, Sajonz P, Guiochon G, J. Chromatogr., 925, 1 (2001)
Li YL, Pinto NG, J. Chromatogr. A, 658(2), 445 (1994)
Martin AJP, Synge RLM, Biochem. J., 35, 1358 (1941)
Guichon G, J. Chromatogr., 969, 129 (2002)
Martin AJP, James AT, Biochem. J., 50, 679 (1952)
Mao QM, Hearn MT, Biotechnol. Bioeng., 52(2), 204 (1996)
Ohinura T, Recombinant human serum albumin process for producing the same and pharmaceutical preparation containing the same, US Patent 5521287 (1996)
Pharmacia LKB biotechnology, Ion exchange chromatography, principle and method (1998)
Wicke E, Kolloid Z, 86(1939)295, citied in Guichon, G. and Lin, B., Modeling for preparative chromatography, Academic press (2003)
Weiss J, J. Chem. Soc., pp. 297, 1943, citied in Guichon, G. and Lin, B., Modeling for preparative chromatography, Academic press (2003)
Wicke E, Kolloid Z, 90(1940)156, citied in Guichon, G. and Lin, B., Modeling for preparative chromatography, Academic press (2003)
Wilson JN, J. Am. Chem. Soc., 62(6), 1583 (1940)

[Referenced By]

[1] Aboudzadeh MR, Jiawen Z, Wu B, Chinese J. Biomedical Eng., 13(4), 166 (2004)

[2] Baker J, Finite element computational fluid mechanics, McGraw-hill, New York (1983)

[3] Bohart GS, Adams EQ, J. Am. Chem. Soc., 42, 523 (1920)

[4] Chen WD, Dong XY, Bai S, Sun Y, Biochem. Eng. J., 14, 45 (2003)

[5] Chung SF, Wen CY, AIChE J., 14, 857 (1968)

[6] Craig LC, J. Biol. Chem., 155,519, 1944, citied in Guichon, G. and Lin, B., Modeling for preparative chromatography, Academic press (2003)

[7] Danckwerts PV, Chem. Eng. Sci., 2, 1 (1953)

[8] De Vault D, J. Am. Chem. Soc., 65(4), 532 (1943)

[9] Foo SC, Rice RG, AIChE J., 21, 1149 (1975)

[10] Foster JF, Rosenoer VM, Oratz M, Rothschild MA, Albumin structure, function and uses, Pergamon Press, 53 (1977)

[11] Geankoplis CJ, Transport processes and unit operations, Allyn and Bacon, MA, 2nd Ed. (1983)

[12] Guiochon G, Golshan-Shirazi S, Jaulmes A, Anal. Chem., 60, 1856 (1988)

[13] Guichon G, Shirazi SG, Katti AM, Fundamentals of preparative and nonlinear chromatography, Academic Press, Boston, MA (1994)

[14] Hirs CHW, Moore S, Stien WH, J. Am. Chem. Soc., 73, 1893 (1951)

[15] Huckman ME, Latheef IM, Anthony RG, AIChE J., 47(6), 1425 (2001)

[16] Kaczmarski K, Antos D, Sajonz H, Sajonz P, Guiochon G, J. Chromatogr., 925, 1 (2001)

[17] Li YL, Pinto NG, J. Chromatogr. A, 658(2), 445 (1994)

[18] Martin AJP, Synge RLM, Biochem. J., 35, 1358 (1941)

[19] Guichon G, J. Chromatogr., 969, 129 (2002)

[20] Martin AJP, James AT, Biochem. J., 50, 679 (1952)

[21] Mao QM, Hearn MT, Biotechnol. Bioeng., 52(2), 204 (1996)

[22] Ohinura T, Recombinant human serum albumin process for producing the same and pharmaceutical preparation containing the same, US Patent 5521287 (1996)

[23] Pharmacia LKB biotechnology, Ion exchange chromatography, principle and method (1998)

[24] Wicke E, Kolloid Z, 86(1939)295, citied in Guichon, G. and Lin, B., Modeling for preparative chromatography, Academic press (2003)

[25] Weiss J, J. Chem. Soc., pp. 297, 1943, citied in Guichon, G. and Lin, B., Modeling for preparative chromatography, Academic press (2003)

[26] Wicke E, Kolloid Z, 90(1940)156, citied in Guichon, G. and Lin, B., Modeling for preparative chromatography, Academic press (2003)

[27] Wilson JN, J. Am. Chem. Soc., 62(6), 1583 (1940)