Influence of preparation parameters on rheological behavior and microstructure of aqueous mixtures of hyaluronic acid/poly(vinyl alcohol)

Hyun-Ok Park; Joung Sook Hong; Kyung Hyun Ahn; Seung Jong Lee; Seong Jae Lee

Korea-Australia Rheology Journal, Vol.17, No.2, 79-85, June, 2005

Hyun-Ok Park, Joung Sook Hong¹, Kyung Hyun Ahn , Seung Jong Lee , and Seong Jae Lee^{2, †}

School of Chemical and Biological Engineering, Seoul National University, San 56-1, Shillim-dong, Gwanak-gu, Seoul 151-744, Korea
¹Applied Rheology Center, Korea University, 5-1, Anam-dong, Seongbuk-gu, Seoul 136-701, Korea
²Department of Polymer Engineering, The University of Suwon, San 2-2, Wau-ri, Bongdam-eup, Hwaseong, Gyeonggi 445-743, Korea

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Aqueous mixtures of hyaluronic acid and poly(vinyl alcohol) system and hydrogels thereof were introduced to obtain new bioartificial materials that have excellent mechanical properties, biocompatibility and enhanced rheological properties. The interactions between hyaluronic acid and poly(vinyl alcohol) and/or borax were investigated by rheological measurements. Preparation parameters of the aqueous mixtures were mixture composition, the degree of hydrolysis of poly(vinyl alcohol) and borax concentration. From the rheological behavior, it could be deduced that the key factor of the interaction between hyaluronic acid and poly(vinyl alcohol) was the hydrogen bonding between them and the effect was pronounced with borax. Enhanced viscosity was observed at the composition of 20 wt% of hyaluronic acid solution and 80 wt% of poly(vinyl alcohol) and borax solution. Rheological properties were influenced by the degree of hydrolysis of poly(vinyl alcohol) and borax concentration. As the degree of hydrolysis and borax concentration increased, rheological properties increased due to the increased hydrogen bonding and networking of hyaluronate aggregates. Physical hydrogels from hyaluronic acid and poly(vinyl alcohol) were prepared and the composition dependence of the gels was rheologically investigated as well.

Keywords:hyaluronic acid;poly(vinyl alcohol);bioartificial material;degree of hydrolysis;hydrogel

[References]

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Hoffman AS, Adv. Drug Deliv. Rev., 43, 3-12 (2002)
Hynes WL, Dixon AR, Walton SL, Aridgides LJ, Fems Microbiol. Lett, 184, 109 (2000)
Kim SH, Hyun K, Mono TS, Mitsumata T, Ahn KH, LEe SJ, Polymer, Morphology-rheology relationship in hyaluronate/poly(vinyl alcohol)/borax polymer blends, Polymer (accepted for publication). (2005)
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Pezron E, Leibler L, Ricard A, Lafuma F, Audebert R, Macromolecules, 22, 1169 (1989)
Prokopova E, Stern P, Quadrat O, Colloid Polym. Sci., 263, 899 (1985)
Ricciardi R, Gaillet C, Ducouret G, Lafuma F, Laupretre F, Polymer, 44(11), 3375 (2003)
Shibayama M, Sato M, Kimura Y, Fujiwara H, Nomura S, Polymer, 29, 336 (1988)
Sinton SW, Macromolecules, 20, 2430 (1987)
Weiss C, Why viscoelasticity is important for the medical uses of hyaluronan and Hylans, in New Frontiers in Medical Sciences: Redefining Hyaluronan, ed. by Abatangelo, G. and P. H. Weigel, 89-103, Elsevier, Amsterdam. (2000)
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[Related Articles]

[1] Ambrosio L, Borzacchiello A, Netti PA, Nicolais L, J. Macromol. Sci. Part A: Pure Appl. Chem., 36, 991 (1999)

[2] Barbucci R, Lamponi S, Borzacchiello A, Ambrosio L, Fini M, Torricelli P, Giardino R, Biomaterials, 23, 4503 (2002)

[3] Cascone MG, Polym. Int., 43, 55 (1997)

[4] Clark G, Staining Procedures, 274-275, Williams & Wilkins, Baltmore. (1981)

[5] Dahl LB, Dahl IM, Enstrom LA, Granath K, Ann. Rheum. Dis., 44, 817 (1985)

[6] Denlinger JL, Hyaluronan and its derivatives as vis-coelastics in medicine, in Chemistry, Biology and Medical Applications Hyaluronan and Its Derivatives, ed. by Laurent, T. C., 235-242, Portland Press, London. (1998)

[7] Finch CA, Polyvinyl Alcohol: Properties and Applications, 41-43, Wiley, London. (1973)

[8] Fouissac E, Milas M, Rinaudo M, Macromolecules, 26, 6945 (1993)

[9] Hoffman AS, Adv. Drug Deliv. Rev., 43, 3-12 (2002)

[10] Hynes WL, Dixon AR, Walton SL, Aridgides LJ, Fems Microbiol. Lett, 184, 109 (2000)

[11] Kim SH, Hyun K, Mono TS, Mitsumata T, Ahn KH, LEe SJ, Polymer, Morphology-rheology relationship in hyaluronate/poly(vinyl alcohol)/borax polymer blends, Polymer (accepted for publication). (2005)

[12] Koopal LK, Colloid Polym. Sci., 259, 490 (1981)

[13] Lozinsky VI, Vainerman ES, Domotenko LV, Mamtsis AM, Titova EF, Belavtseva EM, Rogozhin SV, Colloid Polym. Sci., 264, 19 (1986)

[14] Maerker JM, Sinton SW, J. Rheol., 30, 77 (1986)

[15] Oerther SA, Maurin AC, Payan E, Hubert P, Lapicque F, Presle N, Dexheimer J, Netter P, Lapicque F, Biopolymers, 54, 273 (2000)

[16] Pelletier S, Hubert P, Payan E, Marchal P, Choplin L, Dellacherie E, J. Biomed. Mater. Res., 54, 102 (2001)

[17] Pezron E, Ricard A, Lafuma F, Audebert R, Macromolecules, 21, 1121 (1998)

[18] Pezron E, Leibler L, Ricard A, Lafuma F, Audebert R, Macromolecules, 22, 1169 (1989)

[19] Prokopova E, Stern P, Quadrat O, Colloid Polym. Sci., 263, 899 (1985)

[20] Ricciardi R, Gaillet C, Ducouret G, Lafuma F, Laupretre F, Polymer, 44(11), 3375 (2003)

[21] Shibayama M, Sato M, Kimura Y, Fujiwara H, Nomura S, Polymer, 29, 336 (1988)

[22] Sinton SW, Macromolecules, 20, 2430 (1987)

[23] Weiss C, Why viscoelasticity is important for the medical uses of hyaluronan and Hylans, in New Frontiers in Medical Sciences: Redefining Hyaluronan, ed. by Abatangelo, G. and P. H. Weigel, 89-103, Elsevier, Amsterdam. (2000)

[24] Yamaura K, Shindo N, Matsuzawa S, Colloid Polym. Sci., 259, 1143 (1981)