압전 재료의 탄성표면파 특성과 단백질의 고정화

정우석; 홍철운; 김기범; Woo-Suk Chong; Chul-Un Hong; Gi-Beum Kim

Korean Chemical Engineering Research, Vol.44, No.2, 166-171, April, 2006

Export Citation

압전 재료의 탄성표면파 특성과 단백질의 고정화

Surface Acoustic Wave Characteristics of Piezoelectric Materials and Protein Immobilization

정우석, 홍철운, 김기범^†

전북대학교 공과대학 생체정보공학부, 561-756 전북 전주시 덕진구 덕진동1가 664-14

Woo-Suk Chong, Chul-Un Hong, and Gi-Beum Kim^†

Division of Bionics and Bioinformatics, College of Engineering, Chonbuk National University, 664-14, Duckjin-dong 1ga, Duckjin-gu, Jeonju, Jeonbuk 561-756, Korea

E-mail:

초록

본 연구에서는 전기적 결합 계수가 큰 PMN-PT 압전 재료를 사용하여 탄성표면파를 발진시켜 단백질을 검출할 수있는 새로운 바이오센서로써 이용 가능성을 확인하고자 시도하였다. 실험결과 PMN-PT 압전 재료의 중심 주파수 필터링은 LT 압전 재료보다 우수하였지만, 만족할만한 결과를 얻을 수는 없었다. 또한, 본 연구에서는 위암을 일으키는mismatched DNA를 검출하기 위한 방법을 개발하고자 하였다. 그 결과 EDC 용액을 사용하여 NTA에 MutS를 고정화하였다. 그러나 Ni(니켈)을 사용하여 MutS를 고정화하여 mismatched DNA를 측정하는 것이 더 효과적인 방법이라 판단된다.

In this study, in using a piezoelectric material of Pb(Mg1/3Nb2/3)O3-PbTiO3 (PMN-PT), which has a highelectromechanical coupling coefficient, we have tried to study about this material can be practically available as a newbiosensor to detect protein by using surface acoustic wave (SAW). As the results, the filtering of the center frequency ofthe PMN-PT substrate is a superior result to that of the LiTaO3 (LT) substrate, but the result was not completely satis-factory. Also this study attempts to develop a sensing method to detect mismatched DNA in order to diagnose cancer.We could directly immobilize the MutS to the NTA using the EDC solution. But, we immobilized MutS using nickel andit is judged that is more effective method to detect mismatched DNA.

Keywords:Surface Acoustic Wave Biosensor;Electro-mechanical Coupling Coefficient;PMN-PT;Protein Immobilization

[References]

Wolfbesis OS, Fiber Optic Chemical Sensors and Biosensors, 1, CRC Press, Boca Ration, FL (1991)
Hughes R, Ricco A, Butler M, Martin S, Science, 254, 74 (1991)
Scller FW, Schnbertm F, Fedrowits T, Frontiers in Biosensors, vol. and, Birkhauser, Berlin (1996)
Eggins BR, Biosensors: an Introduction, John Wiley & Sons and B. G. Teubner Publishers, Stuttgart (1996)
Mastrangelo CH, Tribol. Lett., 3, 223 (1997)
Janata J, Josowicz M, Vanysek P, DeVaney DM, Anal. Chem., 70, 179R (1998)
Rogers KR, Biosensors technology for environmental measurment. In: Meyers, R. A., editer, Encyclopedia of Environmental Analysis and Remediation, John Wiley & Sons, New York (1998)
Kim GB, Chong WS, Kwon TK, Hohkawa K, Hong CU, Kim NG, JJAP, 44(4B), 2868 (2005)
Thompson M, Stone DC, “Surface-launched Acoustic Wave Sensors,” John Wiley & Sons, New York (1997)

[Referenced By]

[1] Wolfbesis OS, Fiber Optic Chemical Sensors and Biosensors, 1, CRC Press, Boca Ration, FL (1991)

[2] Hughes R, Ricco A, Butler M, Martin S, Science, 254, 74 (1991)

[3] Scller FW, Schnbertm F, Fedrowits T, Frontiers in Biosensors, vol. and, Birkhauser, Berlin (1996)

[4] Eggins BR, Biosensors: an Introduction, John Wiley & Sons and B. G. Teubner Publishers, Stuttgart (1996)

[5] Mastrangelo CH, Tribol. Lett., 3, 223 (1997)

[6] Janata J, Josowicz M, Vanysek P, DeVaney DM, Anal. Chem., 70, 179R (1998)

[7] Rogers KR, Biosensors technology for environmental measurment. In: Meyers, R. A., editer, Encyclopedia of Environmental Analysis and Remediation, John Wiley & Sons, New York (1998)

[8] Kim GB, Chong WS, Kwon TK, Hohkawa K, Hong CU, Kim NG, JJAP, 44(4B), 2868 (2005)

[9] Thompson M, Stone DC, “Surface-launched Acoustic Wave Sensors,” John Wiley & Sons, New York (1997)