The Allosteric Transition of the Chaperonin GroEL from Escherichia coli as Studied by Solution X-Ray Scattering

Kunihiro Kuwajima; Tomonao Inobe; Munehito Arai

Macromolecular Research, Vol.14, No.2, 166-172, April, 2006

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The Allosteric Transition of the Chaperonin GroEL from Escherichia coli as Studied by Solution X-Ray Scattering

Kunihiro Kuwajima^†, Tomonao Inobe, and Munehito Arai

Department of Physics, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

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This is a short review article of our recent studies on the ATP-induced, allosteric conformational transitionof the chaperonin GroEL complex by solution X-ray scattering. We used synchrotron X-ray scattering with a two-dimensional, charge-coupled, device-based X-ray detector to study (1) the specificity of the chaperonin GroEL forits ligand that induced the allosteric transition, and (2) the identification of the allosteric transition of GroEL in itscomplicated kinetics induced by ATP. Due to the dramatically increased sensitivity of the X-ray scattering techniquebased on the use of the two dimensional X-ray detector and synchrotron radiation, different allosteric conformationalstates of GroEL populated under different conditions were clearly distinguished from each other. It was concludedthat solution X-ray scattering is an extremely powerful tool for investigating the equilibrium and kinetics of coop-erative conformational transitions of oligomeric protein complex, especially when combined with other spectro-scopic techniques such as fluorescence spectroscopy.

Keywords:allosteric transition;chaperonin GroEL;X-ray scattering

[References]

Saibil HR, Horwich AL, Fenton WA, Adv. Protein Chem., 59, 45 (2001)
Sigler PB, Xu ZH, Rye HS, Burston SG, Fenton WA, Horwich AL, Annu. Rev. Biochem., 67, 581 (1998)
Glatter O, Kratky O, Small angle X-ray scattering, Academic Press, London (1982)
Ito K, Amemiya Y, J. Jpn. Soc. Synchrotron Rad. Res., 13, 372 (2000)
Arai M, Ito K, Inobe T, Nakao M, Maki K, Kamagata K, Kihara H, Amemiya Y, Kuwajima K, J. Mol. Biol., 321, 121 (2002)
Kuwajima K, Arai M, Inobe T, Ito K, Nakao M, Maki K, Kamagata K, Kihara H, Amemiya Y, Spectroscopy-Int. J., 16, 127 (2002)
Yifrach O, Horovitz A, Biochemistry, 34, 5303 (1995)
Horovitz A, Curr. Opin. Struct. Biol., 8, 93 (1998)
Inobe T, Makio T, Takasu-Ishikawa E, Terada TP, Kuwajima K, Biochem. Biophys. Acta, 1545, 160 (2001)
Inobe T, Kikushima K, Makio T, Arai M, Kuwajima K, J. Mol. Biol., 329, 121 (2003)
Inobe T, Arai M, Nakao M, Ito K, Kamagata K, Makio T, Amemiya Y, Kihara H, Kuwajima K, J. Mol. Biol., 327, 183 (2003)
Yifrach O, Horovitz A, Biochemistry, 37, 7083 (1998)
Cliff MJ, Kad NM, Hay N, Lund PA, Webb MR, Burston SG, Clarke AR, J. Mol. Biol., 293, 667 (1999)
Svergun D, Barberato C, Koch MHJ, J. Appl. Crystallogr., 28, 768 (1995)
Roseman AM, Chen SX, White H, Braig K, Saibil HR, Cell, 87, 241 (1996)
Inobe T, Kuwajima K, J. Mol. Biol., 339, 199 (2004)
Azem A, Kessel M, Goloubinoff P, Science, 265, 653 (1994)
Llorca O, Marco S, Carrascosa JL, Valpuesta JM, Febs Lett., 345, 181 (1994)
Schmidt M, Rutkat K, Rachel R, Pfeifer G, Jaenicke R, Viitanen P, Lorimer G, Buchner J, Science, 265(5172), 656 (1994)
Corrales FJ, Fersht AR, Folding and Design, 1, 265 (1996)

[Referenced By]

[1] Saibil HR, Horwich AL, Fenton WA, Adv. Protein Chem., 59, 45 (2001)

[2] Sigler PB, Xu ZH, Rye HS, Burston SG, Fenton WA, Horwich AL, Annu. Rev. Biochem., 67, 581 (1998)

[3] Glatter O, Kratky O, Small angle X-ray scattering, Academic Press, London (1982)

[4] Ito K, Amemiya Y, J. Jpn. Soc. Synchrotron Rad. Res., 13, 372 (2000)

[5] Arai M, Ito K, Inobe T, Nakao M, Maki K, Kamagata K, Kihara H, Amemiya Y, Kuwajima K, J. Mol. Biol., 321, 121 (2002)

[6] Kuwajima K, Arai M, Inobe T, Ito K, Nakao M, Maki K, Kamagata K, Kihara H, Amemiya Y, Spectroscopy-Int. J., 16, 127 (2002)

[7] Yifrach O, Horovitz A, Biochemistry, 34, 5303 (1995)

[8] Horovitz A, Curr. Opin. Struct. Biol., 8, 93 (1998)

[9] Inobe T, Makio T, Takasu-Ishikawa E, Terada TP, Kuwajima K, Biochem. Biophys. Acta, 1545, 160 (2001)

[10] Inobe T, Kikushima K, Makio T, Arai M, Kuwajima K, J. Mol. Biol., 329, 121 (2003)

[11] Inobe T, Arai M, Nakao M, Ito K, Kamagata K, Makio T, Amemiya Y, Kihara H, Kuwajima K, J. Mol. Biol., 327, 183 (2003)

[12] Yifrach O, Horovitz A, Biochemistry, 37, 7083 (1998)

[13] Cliff MJ, Kad NM, Hay N, Lund PA, Webb MR, Burston SG, Clarke AR, J. Mol. Biol., 293, 667 (1999)

[14] Svergun D, Barberato C, Koch MHJ, J. Appl. Crystallogr., 28, 768 (1995)

[15] Roseman AM, Chen SX, White H, Braig K, Saibil HR, Cell, 87, 241 (1996)

[16] Inobe T, Kuwajima K, J. Mol. Biol., 339, 199 (2004)

[17] Azem A, Kessel M, Goloubinoff P, Science, 265, 653 (1994)

[18] Llorca O, Marco S, Carrascosa JL, Valpuesta JM, Febs Lett., 345, 181 (1994)

[19] Schmidt M, Rutkat K, Rachel R, Pfeifer G, Jaenicke R, Viitanen P, Lorimer G, Buchner J, Science, 265(5172), 656 (1994)

[20] Corrales FJ, Fersht AR, Folding and Design, 1, 265 (1996)