The primary goal of crystallization process is to generate particles with controlled size, shape and solid form, and the desired chemical purity. Many different types of approaches including molecular level strategies have been devised and employed to control the final structure of crystals. One promising approach is the utilization of self-assembled monolayers (SAMs) as templates. Recently, we reported that single glycine crystals can nucleate on patterned metallic square islands, with the size and morphology of the particles controlled by the dimensions of the islands or the concentration of the solution. Herein, the effect of the solution concentration on the polymorphic outcome of glycine crystals confined to hydrophilic metallic islands is investigated. Furthermore, we examine how variations in the solvent evaporation rate can impact the polymorph distribution of glycine.