A dynamic model has been developed in order to estimate values of the parameters characterizing the interaction of transport phenomena with biospecific adsorption in Bred-bed affinity separations. The derived dimensionless model equations comprise the hydrodynamic behaviour on the basis of the Bodenstein number, influences of mass transfer by the Stanton number and biochemical driving potentials of the adsorption by the Gamma number. Model parameters were estimated on the basis of experimental results obtained from fixed-bed experiments. Results obtained from fixed-bed experiments and model predictions show that transport resistances have an important influence on the efficiency of biospecific separations and have to be considered in the design of large-scale systems as well as in the development of functionalized supports.