Separation of amino acids by thermal parametric pumping is experimentally and theoretically investigated. A multicomponent, non-linear model that takes into account the dissociation reaction in liquid phase is used to predict the separation and to investigate the effect of the resin type on the performance of parametric pumping. Experiments have been done with different mixtures of glutamic acid, leucine, phenylalanine, proline, serine and valine. The experimental results demonstrate the capability of thermal parametric pumping of separating amino acids from aqueous mixtures. The simulations give the correct general behaviour of the experimental results and help to select the resin that is more suitable for the separation.