A cationic and an anionic poly(N-isopropylacrylamide) (poly(NIPAM)) microgel latex were synthesized via batch radical polymerization under emulsifier-free conditions. The hydrodynamic properties, colloidal stability, and electrokinetic characteristics of these two samples were studied. The hydrodynamic particle size variation was discussed by considering the effect of salinity and temperature on the shrinkage of the thermally sensitive polymer domains. The colloidal stability also depended on temperature and electrolyte concentration. A stability diagram with two well-defined domains (stable and unstable) was obtained. The flow from one domain to the other was fully reversible due to the peculiar (de)hydration properties of the polymer. The electrokinetic behavior, which depends on electrical and frictional properties of the particles, was analyzed via electrophoretic mobility measurements. Results were discussed by considering both the particle structure dependence on temperature and salinity, and the electric double layer compression. In addition, the electrophoretic mobility data were analyzed using Ohshima's equations for particles covered by an ion-penetrable surface charged layer, as well as using another simpler equation for charges located on a hydrodynamic equivalent hard sphere. Differences between the properties of both latexes were justified by the presence of a hydrophilic comonomer, aminoethyl methacrylate hydrochloride (AEMH), in the cationic microgel.