Solution-stirring method improves crystal quality of human triosephosphate isomerase

Adachi H; Niino A; Kinoshita T; Warizaya M; Maruki R; Takano K; Matsumura H; Inoue T; Murakami S; Mori Y; Sasaki T

Journal of Bioscience and Bioengineering, Vol.101, No.1, 83-86, 2006

Adachi H, Niino A, Kinoshita T, Warizaya M, Maruki R, Takano K, Matsumura H, Inoue T, Murakami S, Mori Y, Sasaki T

We examined the effect of a solution-stirring method on human triosephosphate isomerase crystallization. The crystals diffracted to more than 1.4 angstrom resolution, whereas those obtained by the normal vapour-diffusion technique diffracted to 2.8 angstrom. These results clearly show that the solution-stirring method is valuable and useful for protein crystallization because of its effectiveness and simplicity.

Keywords:protein crystal growth;solution-stirring;crystal quality;human triosephosphate isomerase;X-ray diffraction