We report the robust attachment of glycosaminoglycans (GAGs) on silanized glass surfaces. Depositions were performed both by immersion and by application of a pattern by means of microcontact printing. Immunofluorescence assays were performed to verify the deposition and the quality of the patterns. In addition, AFM studies of the coated surfaces were performed in order to study some physical characteristics of the deposited GAGs layers. These results may prove useful for the characterization of the mechanical properties of GAGs in the glycocalyx and its relation with cellular migration.