The interaction of the alpha(5)beta(1) integrin with its ligand. fibronectin. Supports numerous adhesive functions and has an important role in health and disease. In recent years, there has been a considerable effort in designing fibronectin-mimetic peptides to target the integrin. However, to date, the therapeutic use of these peptides has been limited, as they cannot accurately mimic fibronectin's binding affinity for alpha(5)beta(1). A peptide-amphiphile (PR_b) was synthesized with a peptide head-roup composed of four building blocks: a spacer; RGDSP, the primary recognition site for alpha(5)beta(1): PHSRN, the synergy binding site; and a linker. The linker was designed to mimic two important criteria: the distance and the hydrophobicity/hydrophilicity between PHSRN and RGD in fibronectin. Human umbilical vein endothelial cells were seeded oil different substrates and evaluated in terms of adhesion, spreading. specificity. cytoskeleton organization, focal adhesions, and secretion of extracellular fibronectin. This peptide was shown to perform comparably to fibronectin. indicating that a biomimetic approach can result in the design of novel peptides with therapeutic potential for biomaterial functionalization.