Stability Studies of Biodegradable Polymersomes Prepared by Emulsion Solvent Evaporation Method

Yuhan Lee; Jae-Byum Chang; Hong Kee Kim; Tae Gwan Park

Macromolecular Research, Vol.14, No.3, 359-364, June, 2006

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Stability Studies of Biodegradable Polymersomes Prepared by Emulsion Solvent Evaporation Method

Yuhan Lee, Jae-Byum Chang, Hong Kee Kim, and Tae Gwan Park^†

Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea

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Di-block copolymers composed of two biocompatible polymers, poly(ethylene glycol) and poly(D,L-lactide), were synthesized by ring-opening polymerization for preparing polymer vesicles (polymersomes). Emulsion solvent evaporation method was used to fabricate the polymersomes. Scanning electron microscope (SEM) images confirmed that polymersomes have a hollow structure inside. Confocal laser microscope and optical microscope were also used to verify the hollow structure of polymersomes. Polymersomes having various sizes from several hundred nanometers to a few micrometers were fabricated. The size of the polymersomes could be readily controlled by altering the relative hydrodynamic volume fraction ratio between hydrophilic and hydrophobic blocks in the copolymer structure, and by varying the fabrication methods. They showed greatly enhanced stability with increased molecular weight of PEG. They maintained their physical and chemical structural integrities after repeated cycles of centrifugation/re-dispersion, and even after treatment with surfactants.

Keywords:self-assembly;polymersome;polymer vesicle;amphiphilic;di-block copolymer;biodegradable

[References]

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[Referenced By]

[1] Antonietti M, Forster S, Adv. Mater., 15, 1323 (2003)

[2] Discher BM, Won YY, Ege DS, Lee JCM, Bates FS, Discher DE, Hammer DA, Science, 284(5417), 1143 (1999)

[3] Warriner HE, Idziak SH, Slack NL, Davidson P, Safinya CR, Science, 271(5251), 969 (1996)

[4] Yu K, Eisenberg A, Macromolecules, 31(11), 3509 (1998)

[5] Nam YS, Kang HS, Park JY, Park TG, Han S, Chang I, Biomaterials, 24, 2053 (2003)

[6] Yokoyama M, Kwon GS, Okano T, Sakurai Y, Seto T, Kataoka K, Bioconjugate Chem., 3, 295 (1992)

[7] Yokoyama M, Okano T, Sakurai Y, Kataoka K, J. Control. Release, 32, 269 (1994)

[8] Gref R, Domb A, Quellac P, Blunk T, Muller RH, Verbavatz JM, Langer R, Adv. Drug Deliv. Rev., 16, 215 (1995)

[9] Yokoyama M, Okano T, Sakurai Y, Suwa S, Kataoka K, J. Control. Release, 39, 351 (1996)

[10] Kwon G, Naito M, Yokoyama M, Okano T, Sakurai Y, Kataoka K, J. Control. Release, 48, 195 (1997)

[11] Kim HK, Park TG, J. Control. Release, 98, 115 (2004)

[12] Yamamoto Y, Yasugi K, Harada A, Nagasaki Y, Kataoka K, J. Control. Release, 82, 359 (2002)

[13] Jule E, Yamamoto Y, Thouvenin M, Nagasaki Y, Kataoka K, J. Control. Release, 97, 407 (2004)

[14] Discher BM, Bermudez H, Hammer DA, Discher DE, Won YY, Bates FS, J. Phys. Chem. B, 106(11), 2848 (2002)

[15] Discher DE, Eisenberg A, Science, 297, 967 (2002)

[16] Meng FH, Hiemstra C, Engbers GHM, Feijen J, Macromolecules, 36(9), 3004 (2003)

[17] Meng F, Engbers GHM, Feijen J, J. Control. Release, 101, 187 (2005)

[18] Yoo HS, ParK TG, J. Control. Release, 70, 63 (2001)

[19] Szoka F, Papahadjopoulos D, Proc. Natl. Acad. Sci. U.S.A., 75, 4194 (1978)

[20] Ahmed F, Discher D, J. Control. Release, 96, 37 (2004)

[21] Gregoriadis G, Liposome Technology, CRC Press, Daejeon (1993)

[22] Jeong JH, Lim DW, Han DK, Park TG, Colloids Surf. B: Biointerfaces, 18, 371 (2000)

[23] Tsutsumi Y, Onda M, Nagata S, Lee B, Kreitman RJ, Pastan I, Proc. Natl. Acad. Sci. U. S. A., 97, 8548 (2000)