High Temperature Size Exclusion Chromatography

Heesook Cho; Soojin Park; Moonhor Ree; Taihyun Chang; Jin Chul Jung; Wang Cheol Zin

Macromolecular Research, Vol.14, No.3, 383-386, June, 2006

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High Temperature Size Exclusion Chromatography

Heesook Cho, Soojin Park, Moonhor Ree, Taihyun Chang^†, Jin Chul Jung¹, and Wang Cheol Zin¹

Department of Chemistry, and Polymer Research Institute, Pohang University of Science and Technology, Pohang 790-784, Korea
¹Department of Materials Sciences and Engineering, and Polymer Research Institute, Pohang University of Science and Technology, Pohang 790-784, Korea

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High temperature size exclusion chromatography (SEC) has been used widely for the characterization of crystalline polymers, for which high temperature operation above the polymer melting temperature is required to dissolve the polymers. However, this high temperature operation has many advantages in SEC separation in addition to merely increasing polymer solubility. At high temperature the eluent viscosity decreases, which in turn decreases the column backpressure and increases the diffusivity of the analytes. Therefore, many reports on the high temperature operation of high performance liquid chromatography (HPLC) have focused on shortening the analysis time and enhancing the resolution. However, the application of high temperature SEC analysis to exploit the merits of high temperature operation is scarce. In this article, therefore, we report on a new apparatus design for high temperature SEC.

Keywords:high temperature;size exclusion chromatography;polystyrene

[References]

Yau WW, Kirkland JJ, Bly DD, Modern Size-Exclusion Liquid Chromatography, Practice of Gel Permeation and Gel Filtration Chromatograph, John Wiley & Sons, New York (1979)
Mori S, Barth HG, Size Exclusion Chromatography, Springer-Verlag, New York (1999)
Choi M, Chung B, Chun B, Chang T, Macromol. Res., 12(1), 127 (2004)
Kim JH, Lee JH, Macromol. Res., 10(2), 54 (2002)
Barth HG, Mays JW, Modern Methods of Polymer Characterization, John Wiley & Sons, New York, Vol. 113 (1991)
Li J, Carr PW, Anal. Chem., 69, 837 (1997)
Xiang Y, Yan B, Yue B, McNeff CV, Carr PW, Lee ML, J. Chromatogr. A, 983, 83 (2003)
Greibrokk T, Andersen T, J. Chromatogr. A, 1000, 743 (2003)
Thompson JD, Carr PW, Anal. Chem., 74, 1017 (2002)
Xiang Y, Liu Y, Lee ML, J. Chromatogr. A, 1104, 198 (2006)
Stoll DR, Carr PW, J. Am. Chem. Soc., 127(14), 5034 (2005)
Li J, Hu Y, Carr PW, Anal. Chem., 69, 3884 (1997)
Dolan JW, Snyder LR, Wolcott RG, Haber P, Baczek T, Kaliszan R, Sander LC, J. Chromatogr. A, 857, 41 (1999)
Vanhoenacker G, Sandra P, J. Chromatogr. A, 1082, 193 (2005)
D'Agnillo L, Soares JBP, Penlidis A, J. Polym. Sci. B: Polym. Phys., 40(10), 905 (2002)
Pasch H, Macromol. Symp., 165, 91 (2001)
Mori S, Katz E, Eksteen R, Schoenmakers P, Miller N, Handbook of HPLC, Marcel Dekker, New York (1998)
Zhu C, Goodall DM, Wren SAC, LC-GC Eur., 17, 530 (2004)
Poppe H, J. Chromatogr. A, 778, 3 (1997)
Renn CN, Synovec RE, Anal. Chem., 64, 479 (1992)
Atkins PW, Physical Chemistry, 6th Ed., Oxford University Press, Oxford (1998)
Harris DC, Quantitative Chemical Analysis, 4th Ed., Freeman, New York (1995)

[Referenced By]

[1] Yau WW, Kirkland JJ, Bly DD, Modern Size-Exclusion Liquid Chromatography, Practice of Gel Permeation and Gel Filtration Chromatograph, John Wiley & Sons, New York (1979)

[2] Mori S, Barth HG, Size Exclusion Chromatography, Springer-Verlag, New York (1999)

[3] Choi M, Chung B, Chun B, Chang T, Macromol. Res., 12(1), 127 (2004)

[4] Kim JH, Lee JH, Macromol. Res., 10(2), 54 (2002)

[5] Barth HG, Mays JW, Modern Methods of Polymer Characterization, John Wiley & Sons, New York, Vol. 113 (1991)

[6] Li J, Carr PW, Anal. Chem., 69, 837 (1997)

[7] Xiang Y, Yan B, Yue B, McNeff CV, Carr PW, Lee ML, J. Chromatogr. A, 983, 83 (2003)

[8] Greibrokk T, Andersen T, J. Chromatogr. A, 1000, 743 (2003)

[9] Thompson JD, Carr PW, Anal. Chem., 74, 1017 (2002)

[10] Xiang Y, Liu Y, Lee ML, J. Chromatogr. A, 1104, 198 (2006)

[11] Stoll DR, Carr PW, J. Am. Chem. Soc., 127(14), 5034 (2005)

[12] Li J, Hu Y, Carr PW, Anal. Chem., 69, 3884 (1997)

[13] Dolan JW, Snyder LR, Wolcott RG, Haber P, Baczek T, Kaliszan R, Sander LC, J. Chromatogr. A, 857, 41 (1999)

[14] Vanhoenacker G, Sandra P, J. Chromatogr. A, 1082, 193 (2005)

[15] D'Agnillo L, Soares JBP, Penlidis A, J. Polym. Sci. B: Polym. Phys., 40(10), 905 (2002)

[16] Pasch H, Macromol. Symp., 165, 91 (2001)

[17] Mori S, Katz E, Eksteen R, Schoenmakers P, Miller N, Handbook of HPLC, Marcel Dekker, New York (1998)

[18] Zhu C, Goodall DM, Wren SAC, LC-GC Eur., 17, 530 (2004)

[19] Poppe H, J. Chromatogr. A, 778, 3 (1997)

[20] Renn CN, Synovec RE, Anal. Chem., 64, 479 (1992)

[21] Atkins PW, Physical Chemistry, 6th Ed., Oxford University Press, Oxford (1998)

[22] Harris DC, Quantitative Chemical Analysis, 4th Ed., Freeman, New York (1995)