화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Clean Technology, Vol.12, No.2, 53-61, June, 2006
Export Citation
초임계 PGSS 법을 이용한 Poly(lactide-co-glycolide)와 단백질의 마이크로복합체 제조에 관한 연구
The Production of Protein-loaded Poly(lactide-co-glycolide) Microparticles using Supercritical Carbon Dioxide
송은석, 정헌섭1, 이한호, 김재덕1, 김화용, 이윤우†
  • 서울대학교 화학생물공학부, 151-742 서울시 관악구 신림9동 산 56-1
  • 1한국과학기술연구원 청정기술연구센터
Eun-Seok Song, Heon-Seop Jung1, Hanho Lee, Jae-Duck Kim1, Hwayong Kim, and Youn-Woo Lee†
  • School of Chemical and Biological Engineering & Institute of Chemical Processes, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul, 151-744, Korea
  • 1Clean Technology Research Center, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, Seongbuk-gu, Seoul, 136-791, Korea
E-mail:
초록
생분해성 고분자인 Poly(lactide-co-glycolide) (PLGA)와 약물 단백질로 약물 중합체를 만들기 위하여 초임계 나노 입자 제조 공정중 하나인 PGSS법을 이용하여 연구를 수행하였다. 초임계 이산화탄소에 의해서 고분자 PLGA의 유리전이온도(Tg)를 강하시켜 가소화시키면서 단백질을 용기 내에서 혼합하였다. 고분자 PLGA에 캡슐화 된 단백질의 입자를 얻기 위해 고압 용기에 들어있는 시료를 노즐을 통하여 대기압으로 이산화탄소를 분사시켰다. 입자의 형태, 입자 크기 그리고 크기 분포에 대한 영향을 알아보기 위해서 고압 용기 안에서 초임계 이산화탄소를 이용하여 공정변수들을 조작함으로서 PGSS 실험을 하였다. 입자는 거칠고 불규칙하며 표면에 기공이 생성되었음을 확인하였다. 제조된 입자의 크기와 크기분포는 과포화도, 핵 생성시간 등으로 설명하였다. 제조된 입자 내의 protein의 활성도 저하는 거의 없었다.
A PGSS (Particles from Gas Saturated Solutions) process designed to generate nano-particles using supercritical fluids has been conducted for the fabrication of Poly(lactide-co-glycolide) (PLGA) microparticles that encapsulate a protein drug. It is demonstrated that the polymer and the dry powder of a protein can be mixed under supercritical carbon dioxide conditions and that the protein component retains its biological activity. In this experiment, the mixture of polymer which is plasticized and dry powder protein was sprayed to form solid polymer that encapsulate the protein. It is found that supercritical fluid process give fine tuning of particle size and particle size distribution by simple manipulations of the process parameters. Porous particles were formed with irregular shape. Protein encapsulated in the polymer was found to have enzymatic activity without significant loss of its initial value.
Keywords:Supercritical CO2;Poly(lactide-co-glycolide);Particles from Gas Saturated Solutions (PGSS);Plasticization;Encapsulation
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