Effect of cooperation between native soil microorganisms and white-rot fungus Bjerkandera adusta R59 on degradation of daunomycin post-production wastes in soil was described. Pure cultures of B. adusta R59 strain were capable to decolorize and decompose that cytostatic xenobiotic in liquid media. Presence of R59 strain in studied daunomycin waste/soil systems increased the rate of the antibiotic conversion. The markers of that process were changes of waste biomass or daunomycin concentration (in pulp) and phenolics level and peroxidase activity (in effluent). It was shown that daunomycin in the wastes may be metabolized even up to 20% of its initial concentration. This effect was conjugated with the propagation of native soil microorganisms (microfungi and bacteria) more significant than in parallel system without R59 strain.