Journal of Chemical Technology and Biotechnology, Vol.81, No.6, 832-840, 2006
Mn-peroxidase production by Panus tigrinus CBS 577.79: response surface optimisation and bioreactor comparison
This study reports the statistical optimisation through response surface methodology of the growth medium for Panus tigrinus manganese-dependent peroxidase (MnP) production in shaken culture. Three crucial variables, including carbon source, malonic acid and Mn2+, were optimised in a nitrogen-limited medium. Sucrose was the best carbon source for MnP production. Mn2+ ions and malonic acid significantly stimulated MnP production at an optimal concentration of 53 mg dm(-3) and 8.2 mmol dm(-3), respectively, resulting in 0.83 U cm(-3). Further experiments were performed in lab-scale stirred tank (STR) and bubble-column (BCR) reactors using the previously optimised liquid medium. BCR proved to be more adequate than STR in supporting MnP production, leading to 3700 U dm(-3) after 144 h with a productivity of 25.7 U dm(-3) h(-1). On a comparative basis with other production data in lab-scale reactors, these results appear to be compatible with scale transfer. (c) 2006 Society of Chemical Industry.
Keywords:Mn-dependent peroxidase production;Panus tigrinus;response surface method;optimisation;shaken cultures;bioreactor