An eight column HPLC-Simulated Moving Bed (SMB) laboratory unit for the separation of enantiomers has been built and operated. The separation of the enantiomers of the Troger's base compound on triacetylcellulose has been considered as model separation. First the thermodynamics of adsorption has been characterised on a single analytical chromatographic column, then the separation has been scaled- up to the SMB laboratory unit. SMB experiments with different operating parameters, i.e., switch time and flow rates, have been run in order to analyse the separation performance of the laboratory unit. The experimental results are discussed in the light of the triangle theory for SMB operation.