The surface of a biomaterial reacts in contact with biological fluids. Hydrogels are used to prepare biomaterials. The surface roughness of materials can be explored by several techniques. However, when considering hydrogels, the surface examined in the dry state does not reflect the final conformation. How the surface roughness is affected by swelling has been little explored by quantitative methods. We have evaluated the surface roughness of poly(2-hydroxyethyl methacrylate) (i.e., pHEMA) by image analysis. Images of disks, prepared from linear pHEMA, were obtained oil a light microscope after various incubation times in saline. Fractal texture analysis was done oil images to determine the fractal dimension D. In this study, D exhibited a significant decrease during swelling and was highly correlated with the swelling ratio (r(2)= 0.994 p < 0.00001). Water uptake by the Surface of the polymer affected the surface roughness. Image analysis using fractal algorithms appears to be the most interesting technique for the quantitative exploration of surfaces of hydrated materials that cannot be measured by conventional methods.