In this paper we report the results of the synthesis and structural, transport, and magnetic characterization of pure La0.5Sr1.5MnO4 and B-site lightly doped samples, i.e. La0.5Sr1.5Mn0.95B0.05O4, where B = Ru, Co, and Ni. The choice was made in order to probe the charge ordering/ orbital ordering ground state of the monolayered La0.5Sr1.5MnO4 manganite as a consequence of the cation doping. It is shown that even a light doping is successful in suppressing the charge and orbital order found in pure La0.5Sr1.5MnO4. No long-range magnetic order has been detected in any of the doped samples but the setup of a spin-glass state with a common freezing temperature (similar to 22 K). Structural parameters show an anisotropy in the lattice constant variation, with the tetragonal distortion increasing as the cell volume reduces, which may suggest a variation in the orbital character of the e(g) electrons along with the overall cation size.