Line imaging of Raman/Rayleigh/CO-LIF is used to investigate the energy and dissipation spectra of turbulent fluctuations in temperature and mixture fraction in several flames, including CH4/H-2/N-2 jet flames at Reynolds numbers of 15,200 and 22,800 (DLR-A and DLR-B) and piloted CH4/air jet flames at Reynolds numbers of 13,400, 22,400, and 33,600 (Sandia flames C, D, and E). The high signal-to-noise ratio of the 1D Rayleigh scattering images enables determination of the turbulent cutoff wavenumber from 1D dissipation spectra. The local length scale inferred from this cutoff is analogous to the Batchelor scale in nonreacting flows. The measured thermal dissipation spectra in the turbulent flames are shown to be similar to the model spectrum of Pope for turbulent kinetic energy dissipation. Furthermore, for flames with Lewis number near unity, the 1D dissipation spectra for temperature and mixture fraction are shown to follow nearly the same rolloff in the high-wavenurnber range, such that the cutoff length scale for thermal dissipation is equal to or slightly smaller than the cutoff length scale for mixture fraction dissipation. Measurements from the piloted CH4/air flames are used to demonstrate that a surrogate cut off scale may be obtained from the dissipation spectrum of the inverse of the Rayleigh signal itself, even when the Rayleigh scattering cross section varies through the flame. This suggests that the cutoff length scale determined from Rayleigh scattering measurements may be used to define the local resolution requirements and optimal data processing procedures for accurate determination of the mean mixture fraction dissipation, based upon Raman scattering measurements or other multiscalar imaging techniques. (c) 2006 The Combustion Institute. Published by Elsevier Inc. All rights reserved.