Urea adduction and high-temperature gas chromatography were used to separate and quantify the amount of normal plus iso-alkanes (n-, iso-)alkanes in 13 neat asphalts. Treating neat asphalt directly with urea eliminated the separation steps necessary for extracting the saturate fraction containing the alkanes from the asphalt. The amounts of n-alkanes including 2-methyl and 3-methyl isomers for the 13 asphalts ranged from 0 to 1.84 wt %. Saturate fractions from three asphalts were obtained by adsorption-desorption chromatography. The amounts of (n-, iso-)alkanes and the highly branched plus cyclic alkanes were determined also for these fractions using urea adduction and were found to be in good agreement with the direct determination of (n-, iso-)alkanes in the neat asphalts. The amount of (n-, iso-)alkanes was found to correlate with the crystalline wax content measured by differential scanning calorimetry, but the method does not account for all of the crystalline wax content. In addition, the amount of (n-, iso-)alkanes does not account for the large n-alkane methylene signals observed in the hydrogen-1 and carbon-13 nuclear magnetic resonance spectra for the asphalts. No correlation was found between the amount of (n-, iso-)alkanes in asphalt and the fracture temperature. However, an apparent correlation does exist between the highly branched plus cyclic alkanes in asphalt and the fracture temperature.