Stability of Immobilized Lipase on Poly(vinyl alcohol) Microspheres

Jae Min Oh; Dong Hwan Lee; Yoon Seok Song; Seung Wook Kim; Se Geun Lee

Journal of Industrial and Engineering Chemistry, Vol.13, No.3, 429-433, May, 2007

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Stability of Immobilized Lipase on Poly(vinyl alcohol) Microspheres

Jae Min Oh, Dong Hwan Lee, Yoon Seok Song, Seung Wook Kim^†, and Se Geun Lee¹

Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea
¹Advanced Nano Materials Research Team, Department of Nano Technology, Daegu Gyeongbuk Institute of Scienceand Technology, Daegu 704-230, Korea

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Candida rugosa lipase was immobilized on poly(vinyl alcohol) microspheres using a cross-linking method. The effects of this immobilization on the thermal and pH stabilities of lipase were investigated. Immobilized lipase showed better pH stability and resistance to acidic environments than free lipase, and higher temperature stability up to 45 ℃. The effect of reuse on the activity of immobilized lipase was also investigated. It was found to retain over 50 % of its original activity after 10 cycles of reuse.

Keywords:immobilization;lipase;poly(vinyl alcohol) microsphere;reuse;stability

[References]

Murty VR, Bhat J, Muniswaran PKA, Biotechnol. Biopro. Eng., 7, 57 (2002)
Schoemaker HE, Mink D, Wubbolts MG, Science, 299, 1694 (2003)
Cha SH, Kim JC, Lim JS, Yoon CS, Koh JH, Chang HI, Kim SW, J. Ind. Eng. Chem., 12(1), 115 (2006)
Balcao VM, Paiva AL, Malcata FX, Enzyme Microb. Technol., 18(6), 392 (1996)
Tischer W, Wedekind F, Top. Curr. Chem., 200, 95 (1999)
Kim DJ, Shin DH, Hur BK, Kim EK, J. Microbiol. Biotechnol., 10, 836 (2000)
Park SW, Choi SY, Chung KH, Hong SI, Kim SW, Biochem. Eng. J., 11, 87 (2002)
Park SW, Kim YI, Chung KH, Kim SW, J. Microbiol. Biotechnol., 11, 199 (2003)
Soares CMF, Santana MHA, Zanin GM, de Castro HF, Biotechnol. Prog., 19(3), 803 (2003)
Byun JW, Lee YS, J. Ind. Eng. Chem., 10(2), 283 (2004)
Lee DH, Park CH, Yeo JM, Kim SW, J. Ind. Eng. Chem., 12(5), 777 (2006)
Lee DH, Kim JM, Kang SW, Lee JW, Kim SW, Biotechnol. Lett., 28, 1965 (2006)
Subramanian A, Kennel SJ, Oden PI, Jacobson KB, Woodward J, Doktycz MJ, Enzyme Microb. Technol., 24(1-2), 26 (1999)
Cho MS, Cho YH, Choi HJ, Jhon MS, Langmuir, 19(14), 5875 (2003)
Lee IS, Cho MS, Choi HJ, Polymer, 46(4), 1317 (2005)
Jin MJ, Yang SH, Han ST, Choe SJ, J. Ind. Eng. Chem., 12(2), 268 (2006)
Alcantara AR, Maria PD, Fernandez M, Hernaiz MJ, Montero JMS, Sinisterra V, Food Technol. Biotechnol., 42, 343 (2004)
Rio JL, Faus I, Biotechnol. Lett., 20, 1021 (1998)
Ulbert O, Belafi-Bako K, Tonova K, Gubicza L, Biocatal. Biotransform., 23, 177 (2005)
Kwon DY, Rhee JS, JAOCS, 63, 89 (1986)

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[2] Schoemaker HE, Mink D, Wubbolts MG, Science, 299, 1694 (2003)

[3] Cha SH, Kim JC, Lim JS, Yoon CS, Koh JH, Chang HI, Kim SW, J. Ind. Eng. Chem., 12(1), 115 (2006)

[4] Balcao VM, Paiva AL, Malcata FX, Enzyme Microb. Technol., 18(6), 392 (1996)

[5] Tischer W, Wedekind F, Top. Curr. Chem., 200, 95 (1999)

[6] Kim DJ, Shin DH, Hur BK, Kim EK, J. Microbiol. Biotechnol., 10, 836 (2000)

[7] Park SW, Choi SY, Chung KH, Hong SI, Kim SW, Biochem. Eng. J., 11, 87 (2002)

[8] Park SW, Kim YI, Chung KH, Kim SW, J. Microbiol. Biotechnol., 11, 199 (2003)

[9] Soares CMF, Santana MHA, Zanin GM, de Castro HF, Biotechnol. Prog., 19(3), 803 (2003)

[10] Byun JW, Lee YS, J. Ind. Eng. Chem., 10(2), 283 (2004)

[11] Lee DH, Park CH, Yeo JM, Kim SW, J. Ind. Eng. Chem., 12(5), 777 (2006)

[12] Lee DH, Kim JM, Kang SW, Lee JW, Kim SW, Biotechnol. Lett., 28, 1965 (2006)

[13] Subramanian A, Kennel SJ, Oden PI, Jacobson KB, Woodward J, Doktycz MJ, Enzyme Microb. Technol., 24(1-2), 26 (1999)

[14] Cho MS, Cho YH, Choi HJ, Jhon MS, Langmuir, 19(14), 5875 (2003)

[15] Lee IS, Cho MS, Choi HJ, Polymer, 46(4), 1317 (2005)

[16] Jin MJ, Yang SH, Han ST, Choe SJ, J. Ind. Eng. Chem., 12(2), 268 (2006)

[17] Alcantara AR, Maria PD, Fernandez M, Hernaiz MJ, Montero JMS, Sinisterra V, Food Technol. Biotechnol., 42, 343 (2004)

[18] Rio JL, Faus I, Biotechnol. Lett., 20, 1021 (1998)

[19] Ulbert O, Belafi-Bako K, Tonova K, Gubicza L, Biocatal. Biotransform., 23, 177 (2005)

[20] Kwon DY, Rhee JS, JAOCS, 63, 89 (1986)