화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Polymer(Korea), Vol.31, No.3, 189-193, May, 2007
Export Citation
PLGA 분자량에 따른 이중층 독소루비신 미립구의 방출거동
Effect of Molecular Weight of PLGA on Release Behavior of Doxorubicin for Double-Layered PLGA Microspheres
박정수, 양재찬, 육순홍1, 신형식2, 이종문, 김문석3, 이해방3, 강길선†
  • 전북대학교 BK-21 고분자 BIN융합연구팀
  • 1한남대학교 고분자공학과
  • 2전북대학교 화학공학과
  • 3한국화학연구원 나노생체재료연구팀
Jung Soo Park, Jae Chan Yang, Soon Hong Yuk1, Hyung Shik Shin2, John M. Rhee, Moon Suk Kim3, Hai Bang Lee3, and Gilson Khang†
  • BK21 Polymer BIN Fusion Res. Team, Chonbuk National University, 664-14, Dukjin, Jeonju 561-756, Korea
  • 1Department of Polymer Science and Engineering, Hannam University, 133, Ojeongdong, Daedeog, Daejeon 306-791, Korea
  • 2Department of Chemical Engineering, Chonbuk National University, 664-14, Dukjin, Jeonju 561-756, Korea
  • 3Nanobiomaterials Lab, Korea Research Institute of Chemical Technology, P.O.BOX 107, Yuseong, Daejeon 305-506, Korea
E-mail:
초록
항암제인 독소루비신의 지속적인 방출을 위하여 PLGA와 독소루비신의 미립구를 수중유형(O/W) 용매증발 방법을 이용하여 약물의 농도와 고분자의 분자량의 변화에 따른 방출거동의 차이를 확인하였다. 이중층 미립구내의 독소루비신의 방출을 분석하기 위하여 형광 분광 광도계를 이용하여 5주 동안 독소루비신의 방출을 보았으며 주사전자현미경을 이용하여 이중층미립구의 단면과 표면을 확인하였다. 제조된 이중층 미립구는 외부층이 전체적으로 매끄러운 표면과 구형을 나타내고 있었고 이중층 미립구의 단면을 잘라 계면층을 중심으로 하여 내부형태와 외부형태를 구분 지을 수 있었다. 또한 제조된 이중층 PLGA 독소루비신 미립구는 방출 결과를 통하여 저분자량의 고분자를 이용할수록 방출이 빠르다는 것을 확인할 수 있었다. 따라서 미립구를 제조하는데 있어서 고분자의 분자량을 조절함으로써 방출거동을 조절할 수 있다는 것을 확인하였다.
We developed the doxorubicin-loaded PLGA double-layered microspheres using relatively simple oil-in-water (O/W) solvent evaporation method for sustained release of doxorubicin and investigated the release behavior according to PLGA molecular weight and initial drug loading. The double-layered microsphere was characterized on the surface, the cross-section morphology, the behavior of doxorubicin release for 5 weeks by SEM and fluorescence spectrophotometer. Double-layered microspheres showed smooth surfaces and clear difference between core and outer-shell. As the PLGA molecular weight increased, the release rate of doxorubicin-loaded, double-layered microspheres decreased. These results showed that the release behaviors can be controlled by the variation of molecular weight of PLGA.
Keywords:dextran;double-layered microsphere;doxorubicin;PLGA
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