마그네슘 존재 하에 형성된 칼슘포스페이트 박막의 물리화학적 특성과 세포증식에 미치는 영향

신현영; 이우걸; Hyun-Young Shin; Woo-Kul Lee

Journal of the Korean Industrial and Engineering Chemistry, Vol.18, No.3, 262-266, June, 2007

Physicochemical Properties of Calcium Phosphate Thin Films Prepared in the Presence of Magnesium and Its Effects on Cellular Proliferation

신현영, 이우걸 ^†

단국대학교 생체계면조직공학 연구실, 화학공학과, 미래과학기술연구소

Hyun-Young Shin, and Woo-Kul Lee^†

Laboratory of Biomaterials & Biointerface Research, Department of Chemical Engineering, Institute of Advances in Science andTechnology, Dankook University, Seoul 140-714, Korea

E-mail:

초록

본 연구에서는 칼슘포스페이트(CP) 박막형성에 있어서 마그네슘 이온의 존재가 박막의 물리화학적 그리고 생물학적 특성에 미치는 영향에 관한 연구를 수행하였다. 실험에서는 5 종류의 다른 표면을 사용하였으며, 배양접시(CTL)와 마그네슘이 존재하지 않는 상태에서 제조한 CP박막(CaP)을 비교군으로 사용하였다. 나머지 3개 종류의 박막은 마그네슘 농도가 다른 조건에서 형성하여 사용하였다. 마그네슘의 농도 0.1, 1 그리고 10 mM 조건에서 형성된 박막을 각각 CaPL, CaPM, 그리고 CaPH로 지정하였다. 제조된 CP박막들의 표면형태를 scanning electron microscopy (SEM)으로 관찰한 결과, 마그네슘의 존재가 박막의 다공성을 감소시키는 등의 상당한 영향을 미치는 것으로 나타났다. 또한 CP박막의 결정성을 분석하기 위해 X-ray diffraction (XRD)로 분석한 결과, 제조된 박막들은 무결정성 구조를 갖는 것으로 나타났다. X-ray photoelectron spectroscopy (XPS) 분석을 통해 형성된 박막은 칼슘, 인, 그리고 마그네슘으로 구성되어 있음을 확인하였다. 제조된 박막에 대한 세포부착실험에서 사용한 마그네슘의 농도가 높을수록 세포부착이 감소하는 것으로 나타났다. 그러나 마그네슘 농도증가에 따라 세포증식이 증가하는 것으로 나타났다. 따라서 이러한 결과는 마그네슘이 존재 하에 CP박막의 생체적합성이 향상될 수 있음을 제시한다.

The effect of the presence of magnesium ions in the preparation of calcium phosphate (CP) thin films on the physicochemical and biological properties of the films has been investigated in this study. Five different surfaces were used and the culture plate (CTL) and CP film prepared in the absence of magnesium (CaP) were used for the comparison. Three different films were prepared at different magnesium concentrations. CP films prepared at the Mg concentrations of 0.1, 1, and 10 mM were designated as CaPL, CaPM, and CaPH, respectively. The observation of surface morphology of the CP films using scanning electron microscopy (SEM) displayed that the presence of magnesium affected considerably the morphology of the films including a decrease of surface porosity. X-ray diffraction (XRD) analysis for the determination of the crystallinity of the CP films showed that the structure of the films was amorphous. Examination using X-ray photoelectron spectroscopy (XPS) confirmed the prepared films consisted of calcium, phophorus, and magnesium. Cell adhesion assays elucidated that cell adhered less as the magnesium concentration increased. However, cell proliferation assays demonstrated that the cells proliferated more actively on the films prepared at the higher magnesium concentration. These results suggest that the presence of magnesium may promote the biocompatibility of CP films.

Keywords:calcium phosphate;thin film;magnesium;osteoblast;XPS;XRD;SEM

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[Related Articles]

[1] Caulier H, van der Waerden JPCM, Paquay YCGJ, Wolke JGC, Kalk W, Naert I, Jansen JA, J. Biomed. Mater. Res., 29, 1061 (1995)

[2] Caulier H, Hayakamwa T, Naert I, van der Waerden JPCM, Wolke JGC, Jansen JA, J. Mater. Sci. -Mater. Med., 8, 531 (1997)

[3] Yuan H, Yang Z, Li Y, Zhang X, de Bruijn JD, Groot K, J. Mater. Sci. -Mater. Med., 9, 723 (1998)

[4] Kizuki T, Ohgaki M, Katsura M, Nakamura S, Hashimoto K, Toda Y, Udagawa S, Yamashita K, Biomaterials, 24, 941 (2003)

[5] Yoshikawa T, Ohgushi H, Tamai S, J. Biomed. Mater. Res., 32, 481 (1996)

[6] Hardy DCR, Frayssinet P, Delince PE, Eur. J. Orthop. Surg. Traumatol., 9, 75 (1999)

[7] Lusquinos F, Pou J, Arias JL, Boutinguiza M, Leon B, Perez-Amor M, Driessens FCM, J. Mater. Sci. -Mater. Med., 13, 601 (2002)

[8] Mo MJ, Shin HY, Jung IH, Ko JS, McGuire J, Lee WK, J. Ind. Eng. Chem., 11(2), 261 (2005)

[9] Clemens JAM, Wolke JGC, Klein CPAT, Groot K, J. Biomed. Mater. Res., 48, 741 (1999)

[10] Torrisi L, Forti G, Appl. Surf. Sci., 69, 140 (1993)

[11] Brugge PJ, Wolke JGC, Jansen JA, J. Biomed. Mater. Res., 60, 70 (2002)

[12] Hulshoff JEG, van Dijk K, de Ruijter JE, Rietveld FJR, Ginsel LA, Jansen JA, J. Biomed. Mater. Res., 40, 464 (1998)

[13] Kokubo T, Kim HM, Kawashita M, Biomaterials, 24, 2161 (2003)

[14] Ding SJ, Ju CP, Lin JHC, J. Biomed. Mater. Res., 47, 551 (1999)

[15] Bigi A, Boanini E, Bracci B, Facchini A, Panzavolta S, Segatti F, Sturba L, Biomaterials, 26, 4085 (2005)

[16] Elin RJ, Clin. Chem., 33, 1965 (1987)

[17] Tsuboi S, Nakagi H, Ishiguro K, Kondo K, Mukai M, Robinson C, Weatherell JA, Calcif. Tissue Int., 54, 34 (1994)

[18] Aizawa M, Terado T, Howell FS, Itatani K, Mater. Res. Bull., 34, 1215 (1999)