A series of glass treatments were investigated to develop a miniaturized enzyme-linked immunosorbent assay microarray sensor with covalently immobilized antibody. Two glass compositions were photo-hydrolytically treated and functionalized with an amino- or mercaptosilane. Surface characterization was done using contact angle, X-ray photoelectron spectroscopy and atomic force microscopy data. Antibody binding efficiency was statistically compared between treatments and glass compositions. Photo-hydrolytic treatment increased surface roughness and hydrophobic behavior due to alkali leaching and molecular surface rearrangement. Antibody binding tests indicated amino functionalized surfaces coupled with photo-hydrolytic treatment adsorb more antibody than those with thiol functionalization and without photo-hydrolytic treatment. (c) 2006 Elsevier B.V. All rights reserved.