The three-dimensional reconstruction of icosahedral viruses from electron micrograph images has become an important tool for understanding virus structure, function, and pathogenesis. We have developed an integrated suite of software programs to automate most of the operations involved in producing these reconstructions from EM images. Our package combines the analytical capabilities of preexisting algorithms together with approaches we have developed to produce an interactive working environment which enhances the efficiency and usefulness of this approach to the structural analysis of icosahedral viruses. (C) 1996 Academic Press, Inc.