We have demonstrated a realistic super-resolution scanning fluorescence microscope using conventional nanosecond lasers. This super-resolution microscope is based on the combination of two-color fluorescence dip spectroscopy and shape modulation to a doughnut beam. Only by introducing a doughnut erase beam, the resolution of the laser fluorescence microscope breaks the diffraction limit by two times without using any mechanical probe. (C) 2003 Elsevier Science B.V. All rights reserved.