Biochips of Au particle arrays were fabricated using nanosphere lithography to investigate the influence of gold roughness upon chemiluminescence induced by peroxidase adsorbed at surface. The corrugation of gold induces a strong enhancement of the chemiluminescence of luminol brought at its vicinity. This letter shows that this enhancement is increased by an order of magnitude when the corrugation is regular and possesses an average curvature radius of around 15 nm (for ordered particles arrays compared to films with random roughness). (c) 2007 Elsevier B.V. All rights reserved.