Emission, excitation, and absorption spectra of 1,4-anthraquinone (1,4-AQ) have been measured in a degassed CCl4 fluid solution at different temperatures. It is shown that 1,4-AQ in CCl4 exhibits weak emission which can be attributed to the S-2(pi, pi*) fluorescence at comparatively high temperature. The S-2(pi, pi*) state is considered to be in thermodynamic equilibrium with the S-1(n, pi*) state as well as with the T-2(n, pi*) and T-1(n, pi*) states. The present observation provides the first example of the S-2 emission from the medium-size molecules containing C=O groups.