Nickel- and copper-modified diamond electrodes were fabricated in view of their application for carbohydrate detection. Both electrodes produced well-defined and reproducible voltammograms for I mM glucose in alkaline media. These electrodes exhibited excellent electrochemical stability with low background current for at least one week in a flow injection analysis system, indicating good adherence of the metal clusters to diamond. These results indicate the promising use of nickel-and copper-modified diamond electrodes for the detection of carbohydrates and amino acids.