Mixed Langmuir-Blodgett (LB) films of arachidic acid (AA) and perfluorotetradecanoic acid (PA) took phase-separated structures with the monolayer domains of AA buried in the monolayer of PA. When two monolayers of AA were transferred on the 1/1 mixed LB films of AA and PA, AA was transferred only on the domains of AA in the mixed LB films.