Chemistry Letters, Vol.32, No.1, 10-11, 2003
DNA protection by PNA from enzymatic digestion for mass-spectroscopic genotyping
By treating single-stranded DNA with nuclease S1 in the presence of peptide nucleic acid (PNA), oligonucleotides of predetermined size were successfully obtained. Mass spectroscopy on these fragments concretely substantiated alteration of one nucleotide to another, if any, in the DNA.