The intercalation of four kinds of hexose for Mg-Al layered double hydroxide (LDH) by the calcination-rehydration reaction using the Mg-Al oxide precursor has been investigated. In spite of non-ionized organic guest, hexose was intercalated for the LDH, and the intercalation behavior of hexose was greatly influenced by the number of axial OH group. The intercalation driving force was thought to be based on hydrogen bond between axial OH group of the intercalated hexose and hydroxide basal layer.