Chemistry Letters, Vol.34, No.11, 1558-1559, 2005
Vanadate as a futile, superoxide ion-producing substrate of trypanothione reductase from Trypanosoma cruzi
Recombinant Trypanosoma cruzi trypanothione reductase can oxidize NADPH in a futile cycle in the presence of vanadate ion as a result of the generation of reactive oxygen and other radical species from the active site dithiol and the NADPH. This process is inhibited by competitive inhibitors of trypanothione reductase catalyzed reduction of trypanothione disulfide.