Journal of Crystal Growth, Vol.210, No.4, 819-823, 2000
Utilization of microgravity to improve the crystal quality of biologically important proteins: chaperonin-60, GrpE, B-subunit of V-type ATPase, and MIF
Protein crystallization experiments under microgravity were performed by using commercial vapor diffusion apparatus (CVDA) hardware on the STS-84 shuttle mission. Chaperonin-60, GrpE, the B subunit of V-type ATPase, and the selenomethionine substituted proteins of macrophage migration inhibitory factor (MIF) were selected as target proteins. The results indicate that microgravity positively affected their crystal growth, supporting the practical utilization of microgravity for the protein structure determination.
Keywords:protein crystallization;microgravity;chaperonin;GrpE;ATPase;macrophage migration inhibitory factor