We report here the growth of Ag film and its thermal stability on the TiO2(110)-(1 x 1) surface using combination techniques of low-energy ion scattering (LEIS), X-ray photoelectron spectroscopy (XPS), and low-energy electron diffraction (LEED). At a surface temperature as low as 125 K, a 2D growth of Ag films seems to occur for submonolayer coverages up to similar to0.8 ML. Annealing of low temperature grown Ag films to 500 K for coverage of 1-2.4 Mt would result in the formation of metastable Ag layers with rest of Ag forming 3D needle-Like islands on top of this Ag film.