This pager describes the synthesis and characterization of low molecular weight cut-off ultrafiltration (UF) membranes from cellulose propionate (CP) polymer using dimethyl acetamide solvent. The casting conditions are studied with reference to changes in the nature of additive and additive to solvent ratio. The membranes are characterized in terms of product permeation rate, solute retention for different electrolytes, as a function of feed concentration and pressure. The retention of different dyes are also included. Molecular weight cut off profiles using polyethylene glycol solutes of different molecular weights are presented. The separation behaviour of oil-water and milk protein systems are presented in order to highlight the fouling resistance of CP membranes.