Diacylglycerol kinase (DGK) phosphorylates the second messenger diacylglycerol (DAG) to yield phosphatidic acid, two neural signaling elements that function to modulate synaptic activity. Of the nine mammalian DGK isotypes known, DGK epsilon (DGK epsilon) shows specificity for arachidonoyldiacylglycerol (20:4-DAG) and selectively contributes to modulate brain signaling pathways linked to synaptic activity and epileptic seizure activity. In this study, we examined changes in gene transcription in a mouse kindling model of epileptogenesis using control DGK epsilon (+/+) and DGK epsilon-knockout (-/-) mice. Total RNA was isolated from the hippocampus and analyzed using RNA and DNA arrays. Significantly altered gene-expression levels were confirmed independently using Western immunoblot analysis. In agreement with our previous studies, a very few number of genes reached a significance of twofold or greater (either up- or down-regulated; p < 0.05). Among the most significantly up-regulated genes in DGK epsilon (+/+) mice included those encoding the inducible prostaglandin synthase cyclooxygenase-2 (COX-2) and tyrosine hydroxylase (TH), also known as tyrosine 3-mono-oxygenase, the rate-limiting enzyme of catecholamine biosynthesis. Kindled DGK epsilon (-/-) animals exhibited no large increases in COX-2 or TH gene expression. These data, plus our previous findings that DGK epsilon (-/-) mice show higher resistance to electroconvulsive shock, suggest an interplay between, and a regulatory role for, DGK epsilon, COX-2, and catecholamine signaling during kindling epileptogenesis. (c) 2005 Elsevier Inc. All rights reserved.